It effectively reduces the body's inflammatory response, and restores the balance between the body's coagulation system and fibrinolytic system 13, 14, 15. Furthermoe, the Ligusticum chuanxiong hort (Chuanxiong) and Dalbergia odorifera (Jiangxiang) can effectively improve microcirculation and reduce blood viscosity 11, 12 Astragalus mongholicus(Huangqi), Pueraria lobata(Gegen), Pseudostellaria sylvatica (Taizishen) can promote blood circulation as well. The Salvia miltiorrhiza (Danshen) in the prescription can expand the coronary artery and resist platelet aggregation, thereby inhibiting the formation of thrombus 10. The differences were statistically significant (P < 0.05) 9. The levels of HDL-C and apoA1 were significantly higher than before treatment. Liu's research showed that chest tightness, chest pain score and serum TC, TG, LDL-C, apoB, and LP(a) levels of patients after treatment with Yixinyin were significantly lower than before treatment. Studies have shown that after patients are treated with Yixinyin, the plasma hsCRP, Fib and D-dimer levels are lower than those of the control group, and there is a significant difference in data between the groups (P < 0.05) 8. Yixinyin has the effect of promoting blood circulation and removing blood stasis, and is clinically used in the treatment of patients with MI 7. With the deepening of research on traditional Chinese medicine, more and more prescriptions are used for the treatment of patients with MI 6. Therefore, the treatment should be based on the adjustment of healthy Qi and the promotion of blood circulation. Traditional Chinese medicine theory believes that Qi deficiency and blood stasis are the pathogenesis of patients with MI. Clinical methods such as drug thrombolysis and coronary stent implantation are commonly used to treat myocardial infarction, but they can relieve symptoms to a certain extent, but cannot repair necrotic myocardial tissue 5. In addition, smoking, diabetes, hypercholesterolemia and hypertension are recognized risk factors related to the occurrence and development of myocardial infarction 4.
The arterial wall response to injury can be induced by a variety of mechanisms, including infection, reactive oxygen species (ROS), oxidized low-density lipoprotein, and hemodynamic shear stress. Excessive inflammation may aggravate heart remodeling and cause heart failure 3. Factors that contribute to plaque vulnerability include the size of the lipid core, the thickness and cell structure of the fibrous cap, and the severity of the inflammatory response. Plaque rupture and subsequent thrombosis is the main cause of symptoms 2. Myocardial infarction (MI) is one of the most common diseases in clinical practice, and the incidence rate has increased significantly in recent years 1. These findings provide a basis for the preliminary research of myocardial infarction therapy in traditional Chinese medicine and provide ideas for the design of related drugs. For that matter, they may be active ingredients of Yixinyin in treating MI. The docking results showed that the components that can be combined with key targets in YixinYin are Supraene, Prostaglandin B1, isomucronulatol-7,2ā²-di-O-glucosiole, angusifolin B, Linolenic acid ethyl ester, and Mandenol. GSEA enrichment analysis revealed that they are mainly involved in pathways associated with myocardial infarction, such as viral myocarditis, VEGF signaling pathway and type I diabetes mellitus. External data sets prove that they behave differently in the healthy and MI (Pā<ā0.05). In the treatment of myocardial infarction, we have obtained key targets of Yixinyin, which are ALDH2, C5AR1, FOS, IL1B, TLR2, TXNRD1. Through molecular docking screening, active components that can combine with key targets in Yixinyin were obtained. GSEA analysis is used to identify the biological processes involved in key targets. We validated the key objectives with external data sets. By integrating differential genes of healthy/MI and unstable angina/MI, key targets of Yixinyin for the treatment of myocardial infarction were screened. These modules can be mapped in Yixinyin PPI network. By constructing a weighted gene co-expression network, gene modules related to myocardial infarction are obtained. In this study, we analyzed gene expression data and clinical information from 248 samples of MI patients with the GSE34198, GSE29111 and GSE66360 data sets. The purpose of our study is to explore the key components and targets of Yixinyin in the treatment of MI. It is often used to treat patients with acute myocardial infarction (MI). Yixinyin, the traditional Chinese medicine, has the effects of replenishing righteous qi, and promoting blood circulation to eliminate blood stagnation.